Prepare for the separation you intend to run
Sample preparation and separation conditions need to be considered together. The supplied SB3 series material lists 2D electrophoresis of membrane and hydrophobic proteins as an application context.
Sample preparation for 2D electrophoresis
Prepare a method-specific evaluation for membrane or hydrophobic protein samples, with solubilization and electrophoresis compatibility considered together.
Conceptual illustration; not experimental data.
Zwitterionic Surfactants
Series-level context
Sample preparation and the two separation dimensions
- Protein
- Formulation / membrane
- Context / optional
Prepare the sample
Prepare the sample
Evaluate solubility and interfering components; include cleanup when the method requires it.
Focus by pI
Focus by pI
First dimension: isoelectric focusing (IEF) separates proteins by isoelectric point.
Equilibrate the strip
Equilibrate the strip
Prepare the focused strip for the second-dimension SDS-PAGE method.
Separate by size
Separate by size
Second dimension: SDS-PAGE separates the focused proteins by molecular weight.
Conceptual sequence only. No gel results are shown. Preparation, focusing and gel conditions require method-specific validation.
- Define the sample challenge
- Record the source, target fraction and preparation history of the membrane or hydrophobic protein sample. Make the preparation question specific enough to compare candidate conditions.
- Start from the complete method
- Review the requirements of the isoelectric-focusing and second-dimension steps, including sample preparation and any cleanup. Do not assume that an extraction condition is automatically suitable for the separation.
- Define a useful comparison
- Set method-specific criteria for sample handling and the resulting separation before running a comparison. Keep preparation conditions and analysis criteria recorded together.
Selection & validation
Check method compatibility before committing
Treat the SB3 entries as candidates for a controlled sample-preparation study, with the final choice made against your electrophoresis method.
- Shortlist by identity and specification
- Compare SB3-12, SB3-14 and SB3-16 using the catalogue and the specification required by your method. Series-level application context is a starting point, not proof that all three will behave the same way.
- Review the full preparation formulation
- Record the candidate concentration, buffer and other preparation components against the requirements of the focusing method. This page supplies neither an IEF-compatible formulation nor a recommended working concentration.
- Validate with appropriate controls
- Compare the planned preparation with your established method and suitable controls. Evaluate the complete separation; no improvement in spots, resolution or protein recovery is claimed here.
Products & support
Select candidates for your preparation study
Review the related SB3 identities and grades, then confirm the specification and documentation needed for your method-specific evaluation.
SB3-12
CAS 14933-08-5
Standard / Super (High Purity) / Ultimate (Cation Controled)
SB3-14
CAS 14933-09-6
Standard / Super (High Purity) / Ultimate (Cation Controled)
SB3-16
CAS 2281-11-0
Standard / Super (High Purity) / Ultimate (Cation Controled)
Discuss your evaluation
Include the sample source, focusing and gel methods, current preparation formulation, candidate material and required documentation. Ask about samples for evaluation without assuming a validated separation outcome.
Bring your application requirements into focus
Share the product, specification, or workflow you are evaluating.